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- 1 -

1D Plot Preferences

- 2 -

2D Plot Preferences

2D Plot Statistics

- A -

Accuri CFlow Files

Importing

Tips for Importing

Activate Gate

Adding a Text Box to a Plot

Adding Custom Data

Adding Signatures

Adding Tokens

Advanced Publishing

Advanced Publishing Preferences

Alerts

Creating

Running

Aligning Objects

Application Button

Applying Compensation Definitions

Applying Gates to Plots

Auto Recovery Files

Axis Options

Axis Scalings

Gating

Quadrants

- B -

batch actions

Folders

Print

Save As Picture

Save Layout

Save to PDF

Save to PowerPoint

Batch Process

Options

Running

Batch Processes

Adding

batch processing

Adding Items to Reports

Adding Pictures to Reports

Report Item Specific Properties

Batch Processing Actions

Batch Processing Reports

Reordering and Copying Items

Batch Processing Tokens

Batch Tab

- C -

Calculating a Single Concentration

Calculating Compensation

Canned Comments

Cell Number Gates

Changing FCS Files

Changing Parameters on Plot Axes

Channel Calibration

Creating

Using

Channel Calibration Tool

Channel Calibration Window

Closing Layouts

Compensation Definitions

Adjusting from a 2D Plot

Applying

Editing

Saving

Setting Up

Compensation Tokens

Compensation Tool

Compensation/Unmixing window

Concentration Calculator

Single Concentration

Statistics Page

Copying

Creating a New Channel Calibration

Creating a New Layout

Creating a User

Creating Alerts

Creating Custom Tokens

Creating Gates

Creating Markers

Creating Security Groups

Current FCS File

Custom Data

Adding/Defining

Deleting

Graphing

Renaming

Working with

Custom Token Formulas

Inserting Functions

Custom Tokens

Boolean Values

Creating

Deleting

Editing

Examples

Formatting

Using Array Formulas

Customizing Default Preferences

Customizing the Ribbon

Cutting

- D -

Data Grids

Fomatting

Parameters to Display

Using Gates

Data List

Adding Data Sets

Changing Data Sets

Sorting

Data Loading

Data Navigator

Data Saving

Data Source

Data Tab

Default Files/Directories

Default Preferences

Customizing

Defining Custom Data

Deleting

Deleting a User

Deleting Custom Data

Deleting Custom Tokens

Deleting Gates

Deleting Markers

Deleting Security Groups

Deleting Signatures

Display Types

DNA Analysis

DNA Histograms

Formatting

Inserting

DNA Position Markers

DNA Preferences

General

Overlays

Position Markers

DNA Statistics

DNA-Specific Tokens

Dockable Windows

Closing

Docking

Opening

Pinning

Undocking

Docking

Docking a Window

Duplicating

Duplicating Security Groups

- E -

Editing a Compensation Definition

Editing a User

Editing Keyword Tokens

Editing Layout Tokens

Editing Security Groups

Editing Statistic Tokens

Editing Tokens

Electronic Signatures

Entering Text

Exporting FCS File Data

Exporting Layouts

PDF

Picture

PowerPoint

Extra Keywords

- F -

FACSDiva Files

Importing

Tips for Importing

FCS Data

Viewing

FCS Data Files

FCS Express Reader

Limitations

Obtaining

Upgrading to FCS Express

FCS File Data

Batch Export

Exporting Single Files

FCS File Headers

Viewing and Changing

FCS File Options

FCS Files

Changing

Current

FCS Version 4

Format Tab

Formatting

2D Plot Specific Options

2D Plots

3D Display Options

Axes

Background

Border and Size

Color Levels

Gate Display

General

Histogram Markers

Histogram Overlays

Histogram Specific Options

Histograms

Legend

Options Shared by All Objects

Options Shared by All Plots

Quadrants

Resolution Options

Title

Formatting DNA Histograms

Background Options

Cycle Options

Formatting Proliferation Histograms

Fit Parameters

Position Markers

Formatting Statistics

Formatting Tables

Formatting Text

Formatting Text Boxes

- G -

Gate Navigator

Gate Preferences

Gate Tokens

Gate View Object

Gates

Applying to Plots

Clipboard

Creating

Deleting

Hiding

Linking

Moving

Using

Gating Tab

General Preferences

Getting Started

Graphing Custom Data

Grouping Items on a Page

- H -

Heat Maps

Color Scheme

Setting Parameters

Well Gates

Working with

Hiding Gates

Hierarchical Gating

Histogram Statistics

Home Tab

- I -

Insert Tab

Inserting Items on a Page

Inserting Plots

Inserting Tables

Inserting Tokens

Installing

Introduction to the Layout

- K -

Keyword Dialog Preferences

Kinetics Analysis

Kinetics Histograms

Inserting

Kinetics Statistics

Kinetics-Specific Tokens

- L -

Layout Navigator

Layout Security Groups

Layout-based Security

Layouts

Closing

Exporting a Page to PowerPoint

Exporting as a PDF

Exporting Page as a Picture

History

Inserting and Deleting Pages

Introduction

Navigating

New

Open

Page Features

Printing Pages

Properties

Publishing

Renaming Pages

Saving

Signing

Unsigning

Viewing Pages

Working with

Zooming

Learning FCS Express

Linking Gates

Linking Markers to Gates

Linking Quadrants to Quadrants

- M -

Magnify

Maintaining Signatures

Managing Password Configurations

Managing the Databases

Markers

Clipboard

Creating

Deleting

Moving

Resizing

Using

Master Page

Using

Modifying Signatures

Moving Gates

Moving Markers

Moving Objects

Moving Quadrants

Multicycle Functions

Multicycle Tab

- N -

Navigating through a Layout

Navigators

- O -

Objects

Aligning

Moving

Ordering

Resizing

Ordering Objects

- P -

Page Layout

Pages

Grouping Items

Inserting Items

Selecting Items

Panels

Using

Working with

Parameter Label Sets

Parameter Math

Creating a Calculation

Creating a Sequence

Picture Plots

Pictures

Inserting

Pinning a Window

Plot Preferences

Plot Statistic Preferences

Plots

Inserting

Magnifying

Types

Preferences

1D Plots

2D Plots

Axis

DNA Cycle Statistics

DNA Experiment Statistics

DNA Model Statistics

Files/Directories

Gates

General

Keyword Dialog

Plot

Plot Statistics

Poliferation Population Statistics

Proliferation Fit Statistics

Save

Security

Startup

Statistics

Proliferation Analysis

Proliferation Fit Parameters

Proliferation Fit Statistic Preferences

Proliferation Histograms

Formatting

Inserting

Proliferation Population Statistic Preferences

Proliferation Postion Markers

Proliferation Statistics

Proliferation-Specific Tokens

Publishing

Advanced

De Novo Software Site

Default Options

Purchasing

- Q -

Quadrants

Clipboard

Moving

Showing

Using

Quick Access Toolbar

Quick Tour of FCS Express

- R -

Regression Analysis

Renaming Custom Data

Resizing Markers

Resizing Objects

Resizing Text Boxes

Ribbon

Customizing

Ribbon Interface

Application Button

Batch Tab

Data Tab

Format Tab

Gating Tab

Home Tab

Insert Tab

Multicycle Tab

Quick Access Toolbar

Text Tab

View Tab

Run

Running Alerts

- S -

Save Preferences

Saving a Layout

Saving Compensation Definitions

Security

Layout-based

User-based

Security Groups

Creating

Deleting

Duplicating

Editing

Security Preferences

Selecting Items on a Page

Selecting the Default Compensation

Setting DNA Preferences

Setting Up Compensation Definitions

Sharing Published Layouts

Showing Quadrants

Signatures

Adding

Deleting

Maintaining

Modifying

Signing Layouts

Stacked Overlay Data

Startup Preferences

Statistic Classifier Tokens

Statistic Preferences

Statistic Tokens

2D Plot Options

Histogram Options

Statistics

2D Plot

Clipboard

Formatting

Histogram

Step 1, label

System Requirements

System Tokens

- T -

Tables

Formatting

Inserting

Text Box Preferences

Text Boxes

Adding Tokens

Entering Text

Formatting

Formatting Text

Inserting

Resizing

Text Tab

Token Properties

Common

Data Source

Display Text

Tokens

Batch Processing

Compensation

Custom

Editing

Gate

Inserting

Inserting a Page Layout Token

Keyword

Layout

Statistic

Statistic Classifier

System

Using

Tutorial

Aligning Objects

Always Display Selection Dialog

Analysis of 3-Cycle Data

Apply Regression to an FCS File

Applying Gates

Applying Gating and Statistics

Autofit Selection (1-Cycle Analysis)

Automatic Compensation

Bar and Scatter Plots

Batch Processing

Cell Concentration

Changing Histogram Overlays

Channel Calibration

Channel Calibration Defaults

Channel Calibration Demonstration

Channel Calibration Tool Setup

Compensation

Complete a Custom Data Grid

Concentration Calculator (Time/Flow Rate)

Concentration Calculator Using Beads

Copying Objects

Create a Linear Regression Token

Create Custom Tokens

Create Token Classifiers

Creating a Classifier Token

Creating a Custom Data Table

Creating a Custom Token

Creating Combination Gates

Creating Custom Data

Creating Gates

Creating the Layout

Cutom Data

Data Navigator

Data Navigator Features

Deleting an Object

Deleting Gates

DNA Analysis

Docking

Docking Features (Multiple Windows)

Duplicating Objects

Excel Report (Cell Mode)

Excel Report (Column Mode)

Expanded Analysis with Autofit

Exporting Plots to PowerPoint

Features of a Docked Window

Fitting Proliferating T-cells

Format to Change Resolution

Formatting 3D Options

Formatting a DNA Histogram

Formatting a Proliferation Histogram

Formatting Legend

Formatting Multiple Plots

Formatting Plot Axes

Formatting Plot Background

Formatting Plot Titles

Formatting Plots

Formatting Text Box Properties

Formatting Text in a Text Box

Formatting using the Pop-up Menu

Gate Navigator

Gate Navigator Features

Gating

Grouping Objects

Insert a Scatter Plot

Insert Plots from Windows Explorer

Inserting

Inserting a 2D Plot

Inserting a Data Grid

Inserting a Histogram

Inserting an Object

Inserting and Modifying a Table

Inserting and Modifying a Token

Inserting Plots

Inserting Text Boxes

Kinetics

Layout Navigator

Layout Navigator Features

Load Layout for Custom Data

Manual Compensation

Marker to Gate Linking

Moving an Object

Moving Gates

Open/Dock Data Navigator

Open/Dock Gate Navigator

Open/Dock Layout Navigator

Ordering Objects

Overlay and Format Kinetics Plots

Pasting Objects

Percentile Quadrants

Plotting Values

Proliferation

Quadrant to Gate Linking

Quadrant/Marker to Gate Linking

Quadrants

Rectangular and Floating Quadrants

Resizing an Object

Save a Regression

Saving and Loading Panels

Saving/Loading Channel Calibrations

Selecting and Deselecting Objects

Selecting Multiple Objects

Setting Bead Gates and Markers

Setting Up a Panel

Setting up Bead Gates

Showing Concentration (Statistics Window)

Showing the Concentration as a Token

Text Boxes and Tokens

Titration

Undocking a Window

Undoing Changes

Using Objects

Using Panels

Using Parameter Math

Using the Panel

Tutorials

- U -

UnMagnify

Unsigning Layouts

User

Creating

Deleting

Editing

User Information

User-based Security

Using Gates

Using Markers

Using Panels

Using Quadrants

Using Templates

Using the Master Page

Using Tokens

- V -

View Tab

Viewing the Raw Data

- W -

Well Gates

Working with Custom Data

Working with Heat Maps

Working with Layouts

Working with Multiple Layouts

Working with Panels

Working with Pictures

Working with Text Boxes

Working with the Data List

- Z -

Zooming a Layout